Association of microsatellite polymorphisms of the GPDS1 locus with normal tension glaucoma in the Japanese population

نویسندگان

  • Kayo Nakamura
  • Masao Ota
  • Akira Meguro
  • Naoko Nomura
  • Kenji Kashiwagi
  • Fumihiko Mabuchi
  • Hiroyuki Iijima
  • Kazuhide Kawase
  • Tetsuya Yamamoto
  • Makoto Nakamura
  • Akira Negi
  • Takeshi Sagara
  • Teruo Nishida
  • Masaru Inatani
  • Hidenobu Tanihara
  • Makoto Aihara
  • Makoto Araie
  • Takeo Fukuchi
  • Haruki Abe
  • Tomomi Higashide
  • Kazuhisa Sugiyama
  • Takashi Kanamoto
  • Yoshiaki Kiuchi
  • Aiko Iwase
  • Shigeaki Ohno
  • Hidetoshi Inoko
  • Nobuhisa Mizuki
چکیده

BACKGROUND To investigate whether the GPDS1 locus, a potential causative locus of pigment-dispersion syndrome, is associated with normal-tension glaucoma (NTG) in Japanese patients. MATERIALS AND METHODS We used polymerase chain reaction amplification with sequence-specific primers to analyze 20 polymorphic microsatellite markers in and around the GPDS1 locus with an automated DNA analyzer and automated fragment detection by fluorescent-based technology. The DNA samples used for these analyses were obtained from ethnicity- and gender-matched patients, including 141 Japanese patients with NTG and 101 healthy controls. Patients exhibiting a comparatively early onset were selected as this suggests that genetic factors may show stronger involvement. RESULTS One allele of D7S2462 exhibited a frequency that was significantly decreased in NTG cases compared to controls (P = 0.0013, Pc = 0.019, OR = 0.48, 95% CI = 0.30-0.75). Alleles at another six microsatellite loci were positively or negatively associated with NTG, but these associations did not retain statistical significance after Bonferroni correction (P < 0.05, Pc > 0.05). CONCLUSION Our study showed a significant association between the GPDS1 locus and NTG, suggesting that there may be some genetic risk factor(s) in the development of NTG.

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عنوان ژورنال:

دوره 3  شماره 

صفحات  -

تاریخ انتشار 2009